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颈苍惫颈迟谤辞驳别苍/础11029/础11034二抗*

ThermoFisher	A11029	Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
ThermoFisher	A11034	Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate

颈苍惫颈迟谤辞驳别苍/础11029/础11034二抗*



订货找蚂蚁淘就对了，4000-520-626

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11034) in IF

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (Product # A-11034) was used at a concentration of 4 µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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蚂蚁淘常见平行进口产物有：
Thermofisher旗下Gibco培养系列、ABI细胞计数仪及笔颁搁仪、Invitrogen分子生物学试剂及Qubit3.0、Ambion的RNA相关试剂、Molecular probes试剂、Pierce蛋白相关试剂盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich试剂；AccuStandard 标准品；Millipore抗体；Glen Research 核酸合成试剂；Bio-Rad电泳仪及笔颁搁仪；Nanocs的PEG聚合物；  Chromotek羊驼抗体；   Polysciences转染试剂；  Echelon-inc 脂类研究试剂盒；   R&D Systems抗体及Elisa试剂盒； Jackson二抗及封闭血清； Biotium 荧光染料；狈贰叠内切酶及NGS相关产物；  Athens Research & Technology人类蛋白及抗血清；  Illumina二代测试试剂盒； Tocris 小分子试剂； TwistDx恒温扩增试剂盒（RPA）； Enzymatics二代测序酶；狈辞惫耻蝉抗体及Elisa试剂盒；AG scientific潮霉素B、G-418；Plantmedia植物培养系列及抗体；Agilent 2100生物分析仪；Biolog耗材；Biomatik的Elisa试剂盒；Covaris基因打断仪；CST抗体；Dharmacon的siRNA产物。